Positional specificity of cyclopropane ring formation from cis-octadecenoic acid isomers in Escherichia coli
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منابع مشابه
Positional specificity of cyclopropane ring formation from cis-octadecenoic acid isomers in Escherichia coli.
An unsaturated fatty acid auxotroph of Escherichia coli was grown with a series of cis-octadecenoate isomers in which the location of the double bond varied from positions 3 to 17. Each of these fatty acid isomers was incorporated into the cellular lipids, but cyclopropane derivatives were formed to at least a 3-fold greater extent from the cis-9 and cis-11 isomers than from any other positiona...
متن کاملPolymorphism in Single-Acid Triglycerides of Positional and Geometric Isomers of Octadecenoic Acid
The polymorphism of 25 glycerol trioctadecenoates with double bonds ranging from ~4-~ I 7 was investigated by differential scanning calorimetry. Triglycerides with cis bonds in odd positions ~7-~13 exhibited three intermediate melting (13'-) forms, but those with cis bonds in even positions, except cis ~4, lacked 13'-forms. Among the trans compounds, only ~ 11, 13, and 14 showed 13'-forms. The ...
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Cyclopropane fatty acids (CPAs) are desirable as renewable chemical feedstocks for the production of paints, plastics, and lubricants. Toward our goal of creating a CPA-accumulating crop, we expressed nine higher plant cyclopropane synthase (CPS) enzymes in the seeds of fad2fae1 Arabidopsis (Arabidopsis thaliana) and observed accumulation of less than 1% CPA. Surprisingly, expression of the Esc...
متن کاملIn vivo distribution and turnover of trans- and cis-10-octadecenoic acid isomers in human plasma lipids.
Triacylglycerols containing deuterium-labeled trans-10- and cis-10-octadecenoic acid (10t-18:1, 10c-18:1) plus the triacylglycerol of deuterated cis-9-octadecenoic acid (9c-18:1) were fed as a mixture to two young, adult male subjects. Analysis by mass spectroscopy of the labeled fats in blood samples collected periodically for 48 h allowed the uptake, distribution and turnover of both 10-octad...
متن کاملCoexpressing Escherichia coli Cyclopropane Synthase with Sterculia foetida Lysophosphatidic Acid Acyltransferase Enhances Cyclopropane Fatty Acid Accumulation1[W][OPEN]
Cyclopropane fatty acids (CPAs) are desirable as renewable chemical feedstocks for the production of paints, plastics, and lubricants. Toward our goal of creating a CPA-accumulating crop, we expressed nine higher plant cyclopropane synthase (CPS) enzymes in the seeds of fad2fae1 Arabidopsis (Arabidopsis thaliana) and observed accumulation of less than 1% CPA. Surprisingly, expression of the Esc...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
سال: 1976
ISSN: 0005-2760
DOI: 10.1016/0005-2760(76)90146-6